Composite

Part:BBa_K4790085:Design

Designed by: Hongyan Jing   Group: iGEM23_NNU-CHINA   (2023-10-11)


ASR5(node 111)/pET-22b(+)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 447
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 366
    Illegal NheI site found at 945
    Illegal PstI site found at 447
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 447
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 447
    Illegal NgoMIV site found at 143
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

The original sequence of pET-22b was downloaded from NCBI. Node 111 was constructed through Ancestral Sequence Reconstruction (ASR).

References